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SQL server CLR creation error "Failed to create AppDomain"

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We have a CLR stored procedure registered on all different SQL environment which was working perfectly. But one SQL server 2008R2 instance throw an exception while creating CLR as:

Failed to create AppDomain "DatabaseName.dbo[runtime].18".

The domain manager specified by the host could not be instantiated.

The same SQL instance was working fine in same days back.

We had tried a lot by repairing .net framework 2.0 and 3.5. Also tried by disabling CLR and drop-created CLR’s again but still the same error.

Is there any server specific configuration need to check and correct?

Pravin Pandit


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